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anti girk4  (Alomone Labs)


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    Structured Review

    Alomone Labs anti girk4
    AnkB Associates With K ACh Channel Subunits in Cardiac Lysates Atrial lysates from AnkB +/− and wild type (WT) mice were evaluated for GIRK1 and <t>GIRK4</t> expression. (A and B) Immunoblots and quantitative analysis of AnkB expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (C and D) Immunoblots and quantitative analysis of GIRK1 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (E and F) Immunoblots and quantitative analysis of GIRK4 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (G to J) Anti-GIRK1 Ig and <t>anti-GIRK4</t> Ig co-immunoprecipitate ankyrin-B from whole heart and atrial lysates. There is no interaction between anti-AnkB and control IgG immunoprecipitates from any lysate. Co-immunoprecipitation (Co-IP) experiments were repeated 3 times. Data are presented as mean ± SEM. Data were analyzed using Mann-Whitney U test (B and D) and unpaired Student's t-test (F); ∗ P < 0.05, ∗∗ P < 0.01.
    Anti Girk4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc+027/Anti-KCNJ5+(Kir3%2E4)+Antibody/pmc13482378-22-10-11
    Average 95 stars, based on 30 article reviews
    anti girk4 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Dysfunction in Ankyrin-B Disrupts Heart Rate Regulation by Altering the Membrane Abundance of GIRK Channels"

    Article Title: Dysfunction in Ankyrin-B Disrupts Heart Rate Regulation by Altering the Membrane Abundance of GIRK Channels

    Journal: JACC: Basic to Translational Science

    doi: 10.1016/j.jacbts.2026.101647

    AnkB Associates With K ACh Channel Subunits in Cardiac Lysates Atrial lysates from AnkB +/− and wild type (WT) mice were evaluated for GIRK1 and GIRK4 expression. (A and B) Immunoblots and quantitative analysis of AnkB expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (C and D) Immunoblots and quantitative analysis of GIRK1 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (E and F) Immunoblots and quantitative analysis of GIRK4 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (G to J) Anti-GIRK1 Ig and anti-GIRK4 Ig co-immunoprecipitate ankyrin-B from whole heart and atrial lysates. There is no interaction between anti-AnkB and control IgG immunoprecipitates from any lysate. Co-immunoprecipitation (Co-IP) experiments were repeated 3 times. Data are presented as mean ± SEM. Data were analyzed using Mann-Whitney U test (B and D) and unpaired Student's t-test (F); ∗ P < 0.05, ∗∗ P < 0.01.
    Figure Legend Snippet: AnkB Associates With K ACh Channel Subunits in Cardiac Lysates Atrial lysates from AnkB +/− and wild type (WT) mice were evaluated for GIRK1 and GIRK4 expression. (A and B) Immunoblots and quantitative analysis of AnkB expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (C and D) Immunoblots and quantitative analysis of GIRK1 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (E and F) Immunoblots and quantitative analysis of GIRK4 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (G to J) Anti-GIRK1 Ig and anti-GIRK4 Ig co-immunoprecipitate ankyrin-B from whole heart and atrial lysates. There is no interaction between anti-AnkB and control IgG immunoprecipitates from any lysate. Co-immunoprecipitation (Co-IP) experiments were repeated 3 times. Data are presented as mean ± SEM. Data were analyzed using Mann-Whitney U test (B and D) and unpaired Student's t-test (F); ∗ P < 0.05, ∗∗ P < 0.01.

    Techniques Used: Expressing, Western Blot, Analysis, Control, Co-Immunoprecipitation Assay, MANN-WHITNEY


    Figure Legend Snippet:

    Techniques Used: Co-Immunoprecipitation Assay, Immunostaining, Western Blot, Patch Clamp

    Related Articles

    other:

    Article Title: Muscarinic acetylcholine receptors M 2 are upregulated in the atrioventricular nodal tract in horses with a high burden of second-degree atrioventricular block.
    Article Snippet: Incubation with primary antibodies (rabbit AntiCHRM2 Antibody, #AMR-002, 1:100, Alomone Labs) diluted in 1% le of Koch (top) were snap frozen and transverse cryosectioning of the AV he bottom image. (B) Masson’s Trichome staining of samples from the CN for the identification of the AV node (C) In the Masson’s Trichome images, rtic valve base of the non-coronary leaflet, which is cartilaginous in some de; CS, Coronary sinus; HCN4, hyperpolarized cyclic nucleotide–gated tricular myocardium.

    Article Title: Spatial Memory Training Counteracts Hippocampal GIRK Channel Decrease in the Transgenic APP Sw,Ind J9 Alzheimer’s Disease Mouse Model
    Article Snippet: GIRK4 , APC-027 , Alomone, Jerusalem, Israel , Rabbit , 1/500.

    Western Blot:

    Article Title: Targeted disruption of the Kcnj5 gene in the female mouse lowers aldosterone levels
    Article Snippet: .. KCNJ5 , Alomone , APC-027 , WB 1/200. ..

    Incubation:

    Article Title: Muscarinic acetylcholine receptors M 2 are upregulated in the atrioventricular nodal tract in horses with a high burden of second-degree atrioventricular block
    Article Snippet: .. The samples were then incubated in primary antibodies [rabbit anti-KCNJ5 (K ir3.4 )/GIRK4, APC-027, 1:100, Alomone Labs] diluted in the same buffer overnight at 4°C. .. For both M 2 and GIRK4 secondary antibodies (Alexa Fluor568-conjugated goat anti-rabbit IgG (1:500, Life Technologies) in 1% BSA was applied for 90 min at room temperature.



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    Image Search Results


    AnkB Associates With K ACh Channel Subunits in Cardiac Lysates Atrial lysates from AnkB +/− and wild type (WT) mice were evaluated for GIRK1 and GIRK4 expression. (A and B) Immunoblots and quantitative analysis of AnkB expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (C and D) Immunoblots and quantitative analysis of GIRK1 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (E and F) Immunoblots and quantitative analysis of GIRK4 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (G to J) Anti-GIRK1 Ig and anti-GIRK4 Ig co-immunoprecipitate ankyrin-B from whole heart and atrial lysates. There is no interaction between anti-AnkB and control IgG immunoprecipitates from any lysate. Co-immunoprecipitation (Co-IP) experiments were repeated 3 times. Data are presented as mean ± SEM. Data were analyzed using Mann-Whitney U test (B and D) and unpaired Student's t-test (F); ∗ P < 0.05, ∗∗ P < 0.01.

    Journal: JACC: Basic to Translational Science

    Article Title: Dysfunction in Ankyrin-B Disrupts Heart Rate Regulation by Altering the Membrane Abundance of GIRK Channels

    doi: 10.1016/j.jacbts.2026.101647

    Figure Lengend Snippet: AnkB Associates With K ACh Channel Subunits in Cardiac Lysates Atrial lysates from AnkB +/− and wild type (WT) mice were evaluated for GIRK1 and GIRK4 expression. (A and B) Immunoblots and quantitative analysis of AnkB expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (C and D) Immunoblots and quantitative analysis of GIRK1 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (E and F) Immunoblots and quantitative analysis of GIRK4 expression normalized to GAPDH in atrial lysates (n = 8 mice/group). (G to J) Anti-GIRK1 Ig and anti-GIRK4 Ig co-immunoprecipitate ankyrin-B from whole heart and atrial lysates. There is no interaction between anti-AnkB and control IgG immunoprecipitates from any lysate. Co-immunoprecipitation (Co-IP) experiments were repeated 3 times. Data are presented as mean ± SEM. Data were analyzed using Mann-Whitney U test (B and D) and unpaired Student's t-test (F); ∗ P < 0.05, ∗∗ P < 0.01.

    Article Snippet: Anti-ankyrin-B Ig (custom antibody produced by Covance), anti-GIRK1 (Alomone Labs), anti-GIRK4 (Alomone Labs), and anti-GAPDH (Fitzgerald Industries Inc).

    Techniques: Expressing, Western Blot, Analysis, Control, Co-Immunoprecipitation Assay, MANN-WHITNEY

    Journal: JACC: Basic to Translational Science

    Article Title: Dysfunction in Ankyrin-B Disrupts Heart Rate Regulation by Altering the Membrane Abundance of GIRK Channels

    doi: 10.1016/j.jacbts.2026.101647

    Figure Lengend Snippet:

    Article Snippet: Anti-ankyrin-B Ig (custom antibody produced by Covance), anti-GIRK1 (Alomone Labs), anti-GIRK4 (Alomone Labs), and anti-GAPDH (Fitzgerald Industries Inc).

    Techniques: Co-Immunoprecipitation Assay, Immunostaining, Western Blot, Patch Clamp